Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

  • Assay type Sandwich-ELISA
  • Format 96T / 48T
  • Test time 3.5 h
  • Detection range 78.13-5000 pg / mL
  • Sensitivity 46.88 pg / mL
  • Sample type and sample volume serum, plasma, and other biological fluids; 100 μL
  • Specificity This kit recognizes rat TNF-α in samples. No significant cross-reactivity or interference was observed between rat TNF-α and analogs.
  • Reproducibility Both intra-CV and inter-CV are <10%.
  • Application This ELISA kit is applied to the quantitative in vitro determination of rat TNF-α concentrations in serum, plasma, and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific for rat TNF-α. The standards or samples are added to the wells of the micro ELISA plate and combined with the specific antibody.

A biotinylated detection antibody specific for rat TNF-α and avidin-horseradish peroxidase conjugate (HRP) are then added successively to each microplate well and incubated. Free components are removed by washing. The substrate solution is added to each well.

Only wells containing rat TNF-α, biotinylated detection antibodies, and avidin-HRP conjugate will appear blue. The enzyme-substrate reaction is terminated with the addition of stop solution and the color turns yellow.

Optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the rat TNF-α concentration. You can calculate the concentration of rat TNF-α in the samples by comparing the OD of the samples with the standard curve.

Kit components and storage

An unopened kit can be stored at 2-8 ℃ for 1 month. If the kit is not supposed to be used within 1 month, please store the components separately according to the following conditions upon receipt of the kit.

Leave a Reply

Your email address will not be published. Required fields are marked *

ABM HSA Mirna inhibitor
anti his hrp Blog culture vessel pakt antibody Product recombinant protein a rtk array wdhd1 western blot image

hsa miR miRNA Inhibitor ABM

Background Non-coding RNAs and significantly microRNAs have been discovered to behave as grasp regulators of most cancers initiation and improvement. The intention of our analysis was to seek out and characterize the carry out of however functionally uncharacterized microRNAs in human breast carcinogenesis. miRNA inhibitors block miRNA regulation of purpose gene expression by safe suppression […]

Read More
HRP Conjugated Arachis
anti his hrp Blog culture vessel pakt antibody Product recombinant protein a rtk array us biological antibodies western blot image

HRP Conjugated Arachis

Preparation Discover Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography. Biochem/Physiol Actions PNA would not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very similar to the anti-T antibody in human sera. […]

Read More
anti his hrp Blog culture vessel cybrd1 pakt antibody Product recombinant protein a rtk array us biological antibodies western blot image

Amyloid Beta Protein

DESCRIPTION Amino Acid Sequence Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val Fundamental description Amyloid β Protein Fragment 1-40 (Aβ40) is derived from the amyloid-β protein (Aβ), which is mapped to human chromosome 21q21.3. Aβ40 is predominantly present inside the vascular amyloid deposits. Aβ40 accommodates of C-terminal membrane insertion space. It reveals structural transition from random coil to a α-helical building in […]

Read More