Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.
Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is obtainable in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) tools (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply.
It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods incorporates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one ingredient peroxidase substrate (SKU: 21530073).
Arachis hypogaea lectin (PNA) |
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| 05-0116-10 | Medicago | 10 mg | EUR 139.2 |
Arachis hypogaea lectin (PNA |
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| 05-0116-1000 | Medicago | 1 g | Ask for price |
Arachis hypogaea lectin (PNA |
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| 05-0116-50 | Medicago | 50 mg | EUR 376.8 |
Arachis hypogaea Arachin Ahy-3 |
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| 1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
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Arachis hypogaea Arachin Ahy-3 |
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| 1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
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Arachis hypogaea Allergen Ara h 1, clone P17 |
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| 1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
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Arachis hypogaea Allergen Ara h 1, clone P41B |
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| 1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
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Arachis hypogaea Allergen Ara h 1, clone P17 |
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| 1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
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Arachis hypogaea Allergen Ara h 1, clone P17 |
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| 1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
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Asprosin Polyclonal Antibody (Human), HRP |
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| 4-PAA332Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Asprosin. This antibody is labeled with HRP. |
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Vinculin (VCL) Polyclonal Antibody (Human), HRP |
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| 4-CAB839Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with HRP. |
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Catalase (CAT) Monoclonal Antibody (Human), HRP |
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| 4-MAC418Hu22-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP. |
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Catalase (CAT) Monoclonal Antibody (Human), HRP |
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| 4-MAC418Hu23-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP. |
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Ferroportin (FPN) Monoclonal Antibody (Human), HRP |
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| 4-MAC489Hu24-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Ferroportin (FPN). This antibody is labeled with HRP. |
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Reelin (RELN) Monoclonal Antibody (Human), HRP |
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| 4-MAC775Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Reelin (RELN). This antibody is labeled with HRP. |
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Reelin (RL) Monoclonal Antibody (Rat), HRP |
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| 4-MAC775Ra21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Reelin (RL). This antibody is labeled with HRP. |
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