Properties
- Assay type Sandwich-ELISA
- Format 96T / 48T
- Test time 2.5 h
- Detection range 3.13-100 ng / mL
- Sensitivity 1.98 ng / mL
- Sample type and sample volume serum, plasma, urine; 50 μL
- Specificity This kit recognizes human RBP4 in samples. No significant interference was observed between human RBP4 and the analogs.
- Reproducibility Both intra-CV and inter-CV are <10%.
- Application This ELISA kit is applied to the quantitative in vitro determination of human RBP4 concentrations in serum, plasma, urine. Consult technical support for applicability if other biological fluids need to be tested.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human RBP4. The samples (or standards) and the biotinylated detection antibody specific for human RBP4 are added to the wells of the micro ELISA plate.
Human RBP4 would combine with the specific antibody. Then, the avidin-horseradish peroxidase (HRP) conjugate is successively added to each well of the microplate and incubated. Free components are removed by washing. The substrate solution is added to each well.
Only wells containing human RBP4, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. The enzyme-substrate reaction is terminated with the addition of stop solution and the color turns yellow. Optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm.
The OD value is proportional to the concentration of human RBP4. You can calculate the concentration of human RBP4 in the samples by comparing the OD of the samples with the standard curve.
Kit components and storage
An unopened kit can be stored at 2-8 ℃ for six months. After testing, unused wells and reagents should be stored according to the table below.
Target Information
Retinol-binding protein 4 (RBP4), formerly called retinol-binding protein (RBP), is a carrier protein. This protein belongs to the lipocalin family and is the specific carrier of retinol (vitamin A alcohol) in the blood [1]. Retinol delivery from liver stores to peripheral tissues.
In plasma, the RBP-retinol complex interacts with transthyretin, preventing its loss by filtration through the renal glomeruli. A vitamin A deficiency blocks the secretion of the binding protein post-translationally and results in faulty delivery and delivery to epidermal cells.
RBP4 appears to be correlated with cardiometabolic markers in chronic inflammatory diseases, including obesity, type 2 diabetes, metabolic syndrome, and cardiovascular disease (CVD) [2].
