Concanavalin A – Canavalia ensiformis

Bio Mag concanvalin a

Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric functions and for gel staining of glycoproteins. In neutral and alkaline choices, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic choices (pH beneath 5.0), Con A exists as a dimer. Con A might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

PRODUCT DESCRIPTION

Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are usually found inside the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

  • Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
  • Detect glycoconjugates in microscopy and circulation cytometry
  • Stain glycoproteins in gels
  • Withstands fixation and permeabilization
  • Number of 10 CF dyes from UV to near-infrared
  • Superior CF dyes are sensible, photostable, and water-soluble

DESCRIPTION Utility

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been moreover used inside the preparation of protein loaded microgels to cease enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Packaging

1 g in poly bottle
25, 100, 250 mg in poly bottle

Biochem/physiol Actions

Con A should not be blood group specific nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for train. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are customary. Con A shows mitogenic train which depends on its diploma of aggregation. Succinylation results in an brisk dimeric sort which stays a dimer above pH 5.6.

Preparation Discover

Extraordinarily purified by specific affinity chromatography.
BioMag Plus
BioMag Plus

Analysis Discover

Agglutination train: Determined from serial dilutions of a 1 mg/mL reply (this train is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.
Concanavalin A (Con A) is probably going one of the crucial usually used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A shows the extreme, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.

Uncover the Correct Stain for Your Utility

Con A and completely different lectins are carbohydrate binding proteins that acknowledge specific sugar moieties on glycoproteins.  The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, completely different cell ground stains or completely different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, might produce greater ground staining and is also additional acceptable in your cell kind. Lectin conjugates might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

 

 

Concanavalin A

T4004-10mg 10mg Ask for price
Description: Concanavalin A

Concanavalin A

T4004-1g 1g Ask for price
Description: Concanavalin A

Concanavalin A

T4004-1mg 1mg Ask for price
Description: Concanavalin A

Concanavalin A

T4004-50mg 50mg Ask for price
Description: Concanavalin A

Concanavalin A

T4004-5mg 5mg Ask for price
Description: Concanavalin A

Concanavalin A

RM4157-100MG 1 unit
EUR 116.59
Description: Concanavalin A

Concanavalin A

RM4157-25MG 1 unit
EUR 33.38
Description: Concanavalin A

Concanavalin A

inh-cona-2 2 x 100 mg
EUR 143.85

Concanavalin A IV

abx082153-25mg 25 mg
EUR 309.6

Concanavalin A IV

abx082153-100l 100 µl
EUR 162.5

Concanavalin A IV

abx082153-1ml 1 ml Ask for price

Concanavalin A IV

abx082153-200l 200 µl Ask for price

Concanavalin A (ConA)

05-0106-100 100 mg
EUR 91.2

Concanavalin A (ConA)

05-0106-1000 1 g
EUR 238.8

Concanavalin A (ConA)

05-0106-10000 10 g Ask for price

Concanavalin A (ConA)

05-0106-100000 100 g Ask for price

Concanavalin A (ConA)

05-0106-250 250 mg
EUR 105.6

Concanavalin A (ConA)

E16FXP010 1 mg
EUR 86.67
Description: Available in various conjugation types.

Concanavalin A ELISA Kit

20-abx052181
  • Ask for price
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  • Ask for price
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Concanavalin A ELISA Kit

ECP7827 96 Tests
EUR 479

Concanavalin A (Con A) Antibody

20-abx101152
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  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Concanavalin A agarose gel

EA-IP-014 2mL
EUR 242
Description: Primary Antibodies

Concanavalin A (ConA) Antibody

abx101152-100l 100 µl
EUR 287.5

Concanavalin A (ConA) Antibody

abx101152-1ml 1 ml
EUR 850

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