Concanavalin A – Canavalia ensiformis

Bio Mag concanvalin a

Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric functions and for gel staining of glycoproteins. In neutral and alkaline choices, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic choices (pH beneath 5.0), Con A exists as a dimer. Con A might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

PRODUCT DESCRIPTION

Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are usually found inside the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

  • Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
  • Detect glycoconjugates in microscopy and circulation cytometry
  • Stain glycoproteins in gels
  • Withstands fixation and permeabilization
  • Number of 10 CF dyes from UV to near-infrared
  • Superior CF dyes are sensible, photostable, and water-soluble

DESCRIPTION Utility

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been moreover used inside the preparation of protein loaded microgels to cease enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Packaging

1 g in poly bottle
25, 100, 250 mg in poly bottle

Biochem/physiol Actions

Con A should not be blood group specific nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for train. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are customary. Con A shows mitogenic train which depends on its diploma of aggregation. Succinylation results in an brisk dimeric sort which stays a dimer above pH 5.6.

Preparation Discover

Extraordinarily purified by specific affinity chromatography.
BioMag Plus
BioMag Plus

Analysis Discover

Agglutination train: Determined from serial dilutions of a 1 mg/mL reply (this train is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.
Concanavalin A (Con A) is probably going one of the crucial usually used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A shows the extreme, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.

Uncover the Correct Stain for Your Utility

Con A and completely different lectins are carbohydrate binding proteins that acknowledge specific sugar moieties on glycoproteins.  The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, completely different cell ground stains or completely different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, might produce greater ground staining and is also additional acceptable in your cell kind. Lectin conjugates might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

 

 

Concanavalin A (ConA)

05-0106-250 250 mg
EUR 88

BioMagPlus Concanavalin A

BP531-10 10 ml
EUR 1166.44
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

BioMagPlus Concanavalin A

BP531-3 3 ml
EUR 518.29
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

Concanavalin A IV

abx082153-25mg 25 mg
EUR 258

Concanavalin A (Con A) Antibody

20-abx101152
  • EUR 272.00
  • EUR 133.00
  • EUR 676.00
  • EUR 342.00
  • EUR 244.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Concanavalin A, CF350 conjugate

29015 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF488A conjugate

29016 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF594 conjugate

29017 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF633 conjugate

29018 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF640R conjugate

29019 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF680 conjugate

29020 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF770 conjugate

29058 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF405M conjugate

29074 5x1mg
EUR 219
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF405S conjugate

29075 5x1mg
EUR 219
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF750 conjugate

29080 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A ELISA Kit

20-abx052181
  • EUR 6439.00
  • EUR 3432.00
  • EUR 801.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Concanavalin A (ConA) CLIA Kit

20-abx190017
  • EUR 7645.00
  • EUR 4074.00
  • EUR 942.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Concanavalin A (ConA) Antibody Pair

20-abx370219
  • EUR 1400.00
  • EUR 899.00
  • 10 × 96 tests
  • 5 × 96 tests

Concanavalin A (ConA) ELISA Kit

20-abx150329
  • EUR 6047.00
  • EUR 3228.00
  • EUR 754.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Concanavalin A (ConA) Antibody (Biotin)

20-abx271751
  • EUR 384.00
  • EUR 230.00
  • EUR 1052.00
  • EUR 523.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Concanavalin A ELISA kit

E02C0795-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit

E02C0795-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit

E02C0795-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Concanavalin A ELISA kit

E03C0795-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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